AVALIAÇÃO DA AÇÃO ANTIMICROBIANA DE ÓLEOS ESSENCIAIS SOBRE BACTÉRIAS DE IMPORTÂNCIA MÉDICA
AVALIAÇÃO DA AÇÃO ANTIMICROBIANA DE ÓLEOS ESSENCIAIS SOBRE BACTÉRIAS DE IMPORTÂNCIA MÉDICA
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DOI: https://doi.org/10.22533/at.ed.011112620012
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Palavras-chave: atividade antibacteriana, cravo botão, Staphylococcus aureus
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Keywords: antibacterial activity, clove bud, Staphylococcus aureus
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Abstract: Antimicrobial resistance represents one of the major challenges in contemporary public health, driving the search for alternative therapeutic approaches. In this context, essential oils—volatile secondary metabolites extracted from aromatic plants—have gained attention due to their antimicrobial properties. This study aimed to evaluate the antimicrobial activity of essential oils from Eucalyptus globulus, Tea Tree (Melaleuca alternifolia), and Clove Bud (Eugenia caryophyllus), used individually and in binary combinations, against clinically relevant bacteria. The oils were applied in their pure form and in combinations (Eucalyptus + Clove, Eucalyptus + Tea Tree, and Tea Tree + Clove), impregnated onto 6 mm filter paper discs, and placed on Petri dishes containing culture medium previously inoculated with Pseudomonas aeruginosa, Proteus mirabilis, Enterobacter aerogenes, Klebsiella pneumoniae, Shigella flexneri, Salmonella Typhimurium, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, and Enterococcus faecalis. The plates were incubated at 35 °C for 24 to 48 hours, and antimicrobial activity was assessed by the formation of inhibition zones. The results demonstrated greater efficacy of Clove Bud oil in its pure form, particularly against S. aureus, S. epidermidis, and S. flexneri. Tea Tree oil showed intermediate activity, whereas Eucalyptus globulus exhibited limited antimicrobial action. The combinations displayed synergistic effects, especially Eucalyptus globulus + Clove Bud, which produced the largest inhibition zone against S. aureus (46 mm). Resistance of P. aeruginosa to most treatments was observed. These findings indicate the antimicrobial potential of essential oils as a complementary alternative for the control of microorganisms.
- Maria Luiza Silva Fazio
- Amanda Rossi Ribeiro
- Gabriel Bobadilha Braga
- Maria Luisa Souza Marques